(D) qRT-PCR of animal pole explants examining the fold change from stage 9 to 13 of expression of mesodermal marker Bra(T) for epidermis(WT), treated with BMP4/7(20ng/uL) at stage 9 and treated with BMP4/7 (20ng/uL) at stage 10.5 (*** P<0.005).Īdditional file 8: Supplemental Figure 8. Gel was cropped to show one replicate, uncropped gel is Supplemental Figure 9B. (C) Western Blot Analysis of lysates for epidermal (WT) and BMP4/7 treated at stage 9 (BMP4/7 20ng/uL) explants collected at stage 10 and epidermal (WT) and BMP4/7 treated at stage 10.5 (BMP4/7 20ng/uL) explants collected at stage 11 for pSmad1/5/8 with Actin loading control. (B) Number of enriched Mesoderm GO Terms (solid line) and Epidermis GO Terms (dashed line) in genes significantly higher in the ventral mesoderm lineage (purple) and epidermal lineage (red) at each developmental stage. (A) Number of differentially expressed genes between the epidermal and ventral mesoderm lineages at each developmental stage (padj ≤ 0.05). Early not Exogenous BMP Causes Mesoderm Formation. The Creative Commons Public Domain Dedication waiver ( ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.Īdditional file 7: Supplemental Figure 7. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made.
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